Doc Oct's lab is first shown to be a regular building on a street, but it is later shown to be located on a dock. [Two different labs.]

When Dr Octavius first meets Peter he says, "Intelligence is not a privilege - it's a gift to be used for the good of mankind." But in the scene towards the end of the movie where Doc Ock has realized that Spiderman is Peter Parker, Peter says, "You once spoke to me about intelligence, that it was a gift to be used for the good of mankind." Doc Ock nods his head and says, "A privilege." He's contradicting himself. [Character mistake - he misremembers. Not surprisingly, considering the stress he's been under lately.]

In the new "2.1 extended edition" there is a scene where Spider-Man and Doc Ock are fighting on the side of the train and people on the loading platform have to scatter to avoid being hit. But if you pay attention, the train is on the opposite track that it is on in both previous and following shots. [In the features on the 2.1 DVD, the producers say that the theatrical release will always be the "real" Spider-Man 2, and that 2.1 is just something they put together for the fans. The extra shots in the train scenes was taken from footage discarded early in original production, so they had to basically put all the effects together from scratch using spare background and coverage shots. A few slight mistakes would be expected.]

In the scene when Doc Ock picks up Spiderman's aunt in front of the bank, just before one of his arms grab her, one can see Spidey's aunt has already been lifted up. [If you look closely you can see that a smaller tentacle comes out to pick her up.]

During the fusion process, all metal objects are drawn to the energy source, yet Octavius' wife's earrings aren't affected at all. [Not all metals are subject to magnetism. Gold and silver are not.]

The Spider-Man outfit that Peter throws in the trash appears to either have the head attached to the body or a very long neck on the mask. It hangs too far down over the edge of trash can to be the same, separate headpiece, with a short neck, that Jameson receives from the trash collector. (Though this may be a deliberate nod to the comic book, it is still a mistake.) [Jameson must have tucked the neck up inside the mask so it would hang correctly on the wall. If he hadn't, the extra material would have bunched up under the chin area and pushed the face upward. Jameson clearly wanted the mask to "look" straight ahead.]

In the stopped subway car, when the crowd is passing the unmasked Spider-Man over their heads, the pose of Spider-Man and camera angle used are the same as the death scene done by Kenneth Branagh in his four hour "Hamlet" (1996). [Purely coincidental, not trivia.]

Throughout the film you can see that Spiderman is hanging with his web from nothing, just from the sky. One really obvious is when you see him in the night, before he starts loosing his powers. He's not hanging from the buildings, but from the sky. [The web goes off camera so we don't see what it's attached to. He's in New York so it could easily be attached to a very high point on one of the buildings. From a practical standpoint this would make it easier for him to swing straight down a street, rather than at angles if he attached the web lower on the buildings.]

When you look at Spider-Man's skin when his suit has been damaged, you can see that it is fake skin. You can see this when the passengers of the train held Spider-Man just after he fainted. [I've watched the scene several times and there's no way to determine his skin is fake.]

When Spiderman loses his powers, he falls from the building having just tried to climb up, and grabs a newspaper. In this shot he still has his gloves on, but when he grabs the paper and focuses his gloves are off. [This is not a mistake, this is a transition from the alley to Peter's apartment when he does not have his costume on. You see it from his point of view, as he holds it then throws it, then it cuts to a wideshot of his apartment.]

In the scene where Mary Jane is performing in her play, and notices that Peter shows up, she says "Yes. I mean, no" when the other actresses asks about forgiving. The "I mean, no" part is actually in The Importance Of Being Earnest" (the play), even though it looks like Mary Jane was so annoyed with Peter that she messed up her line at first. [The fact that the line was correct is not really trivia worthy. As you have pointed out the line was in the play which means Mary Jane's reaction and speaking of the line were spot on so nothing really out of the ordinary.]

After the German girl and Peter eat chocolate cake, and she gives him a message, when she says, "It's from your aunt," she has almost no accent in her voice. [Two things, first the girl is Russian, not German, and second, she never talks with an accent prior to this moment, so she may very well just not have one.]

When Peter is delivering the pizzas to the receptionist, she can be seen sipping her drink through her straw. The straw can be seen in the container, but not in the liquid as she sips. [She might just be trying to push the straw up and down the bottle to find the liquid because we eventually do hear the slurping sound from water going through the straw.]

When the train scene first starts, it shows Doc Ock and Spider-Man landing on the front of the train and rolling back denting the roof of the train. But when Doc Ock goes to the front of the train later to rip out the speed control, there are no dents on the roof. [They don't land on the front, which is why Doc Ock has to walk up to the front.]

When Peter transforms into Spiderman to get to his next delivery quicker, two kids run out into the street. The kids reach the curb, then from Spiderman's perspective, they reach the curb again. [These "repeat scenes" are common movie making/editing techniques. Its purpose is to show exactly what you’ve just pointed out, to show two scenes shown from two different “points of view”. These are not movie mistakes.]

When Peter is on his way to see MJ in the beginning the police are chasing two bad guys. When the bad guys shoot at the police car, it swerves. The following shot we see from the perspective of the other police car it swerves again. [It's not highly unusual for a car to swerve more than once during a car chase.]

When Aunt May and Peter are talking, while she is packing up her things in her backyard, the cream-colored Oldsmobile can be seen in the garage. This is the same car that was stolen and totalled by the thief that killed Uncle Ben in Spider-Man. Even if the smashed up hulk of the car was retained by May Parker, her financial situation would never have allowed her to get it restored/rebuilt to the condition it is in. And no insurance company would have come close to repairing a car of that vintage. The car should not be there. [It is impossible to claim that the car "should not be there" when there are a variety of suitable explanations available. She could have indeed paid for the repairs herself for sentimental reasons, perhaps she used insurance money from Uncle Ben's death, or she sold something, or she did it with Peter's assistance, or the car insurance company may very well have paid for repairs. It is unnecessary to figure out how the car was repaired, it simply was. Also, as stated in trivia, the Oldsmobile is Sam Ramie's trademark.]

When getting dressed to go to M.J.'s play, there is a cute scene showing Peter staring into his closet, looking back and forth in the closet, trying to decide what to wear. A reverse angle of the closet's contents shows that there are only two items hanging there, the 'Spidey' suit, and Peter's one-and-only dress shirt/coat/ tie. The gag, of course, is that he is trying to decide what to wear, when it is obvious that he is going as a 'civilian', so he should wear the street clothes. However, after the play is over, Peter (who logically chose to wear his street clothes) is shown running down an alley, and he does the 'opening the shirt to reveal the 'Spidey' suit underneath' maneuver, and swings away on a web. Well.if he wears the costume underneath his street clothes most of the time, why was he looking in the closet trying to decide between the two of them when he knew he was going to be wearing them both? [The gag isn't "deciding what to wear", it's supposed to show how he's struggling to decide whether he should wear his Spidey suit underneath or not, as he's still wondering whether he should give up being Spiderman or not. It's a tougher decision than it seems because if he DOES wear the Spiderman suit underneath his normal suit, then he's under pressure to go off and be Spiderman if he sees anything happening on his way there (and thus disappointing MJ) but if he doesn't wear it, there's no way he can go off to save people anyway, so he'd make MJ happy, but potentially cause other people to die (as he did with his uncle). It's a lot deeper than a simple "he can't decide what to wear" gag.]

After Peter decides not to be Spider-man anymore, he's walking as a normal person, trips and falls. There are trees and a park bench in the surrounding area where he falls. Yet when he gets up, he is on a sidewalk in front of a building with no trees or park bench anywhere. [Notice that there are several shots of him falling. This is to indicate that he has tripped several times in several locations; he is in a completely different area when he is finally seen standing.]

The first movie established that Peter was mutated with spider DNA, that the changes in him were at a molecular level. Considering this, even a high level of stress should not be able to reverse a change that was imprinted in is own DNA marking. We're not talking insomnia or losing balance here (which *can* be caused by stress): saying that he lost his eyesight, his little grabbing hairs and his web-making capacities is like saying that because I'm stressed, my eyes will change colors, my arms will fall off or I'll stop salivating. All at once. Just not possible. [As the doctor explains, Peter's problem is psychological. Think of it like a major league baseball player who's so stressed out he loses his confidence in himself and can't home runs anymore. His body hasn't changed, but it can't perform for him as well b/c of mental problems.]